BACGroTM Baird-Parker Agar Base, supplemented with Egg Yolk Tellurite Enrichment, is intended for the selective isolation and enumeration of Staphylococcus aureus from food products and other materials. Baird-Parker Agar Base is not intended for use in diagnosis, treatment, or prevention of disease in humans.
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The Baird-Parker Agar Base is used as a selective enumeration medium for Staphylococcus aureus. It is adopted by AOAC International as an Official Method of Analysis for S. aureus.
The combination of peptone, yeast extract, and beef extract provide nitrogen, carbon, and trace vitamins and minerals to support the growth S. aureus. The inclusion of sodium pyruvate further supports the growth on S. aureus in the presence of selective agents. The egg yolk additive produces a halo around strains of S. aureus owing to the production of lecithinase, allowing for differentiation from other species of Staphylococcus. The inclusion of glycine and lithium chloride provide selectivity against other organisms, including Gram negative and other Gram positive bacteria. The addition of tellurite in the Egg Yolk Tellurite enrichment provides further specificity, and also results in a black color for S. aureus colonies.
Casein Peptone 10.0 g
Yeast Extract 1.0 g
Beef Extract 5.0 g
Sodium Pyruvate 10.0 g
Glycine 12.0 g
Lithium Chloride 5.0 g
Agar 20 g
Total 63 g/L
