BACGroTM Rappaport Vassiliadis (RV) Broth is a selective enrichment medium used in the isolation of Salmonella. BACGroTM RV Broth conforms to ISO 11133:2014 for the isolation of Salmonella.
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Rappaport Vassiliadis Broth is used in the isolation of Salmonella from nonsterile food goods. Soy peptone provides nitrogen. Potassium phosphate acts as a buffer, providing a low pH to inhibit competitive organisms. Sodium chloride maintains osmotic pressure. Malachite green is inhibitory to other organisms other than Salmonella. Some serovars of Salmonella, including Typhi and Enteritidis, may be susceptible to malachite green; if these organisms are suspected to be present, an alternative enrichment broth should be used in parallel. All results should be subcultured to XLD to confirm growth.
Soy Peptone 4.5 g/L
Sodium Chloride 8.0 g/L
Potassium Phosphate, Monobasic 0.6 g/L
Potassium Phosphate, Dibasic 0.4 g/L
Magnesium Chloride, Anhydrous 13.58 g/L
Malachite Green 0.036 g/L
Total 27.1 g/L
