BACGroTM Hektoen Enteric Agar (HE) is a selective and differential medium used in the isolation and differentiation of Gram-negative enteric pathogens.
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Hektoen Enteric agar utilizes bile salts to inhibit Gram-positive organisms and some Gram-negative strains. Optimized differentiation is achieved through the addition of three carbohydrates: lactose, sucrose, and salicin. Ferric ammonium citrate and sodium thiosulfate allow for the detection of hydrogen sulfate production, aiding in differentiation by causing black-centered colonies for Salmonella.
Acid fuchsin and Bromothymol blue is an indicator system with a lower toxicity to promote improved enteric pathogen recovery.
Animal Peptone 12.0 g
Yeast Extract 3.0 g
Bile Salts 9.0 g
Lactose 12.0 g
Sucrose 12.0 g
Salicin 2.0 g
Sodium Chloride 5.0 g
Sodium Thiosulfate 5.0 g
Ferric Ammonium Citrate 1.5 g
Bromothymol Blue 0.064 g
Acid Fuchsin 0.1 g
Agar 13.5 g
Total 75.2 g/L
