BACGroTM MacConkey Agar w. Sorbitol (sMAC), when prepared as directed, is used as a partially selective and differential media for the isolation of E. coli O157:H7. It is not intended for use in diagnosis, treatment, or prevention of disease in humans.
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All customers shall contact BACGroTM Culture Media directly via e-mail or phone +1-215-357-3911.
The formulation of BACGroTM sMAC is comprised of peptones to provide nutrient sources. D-sorbitol is a fermentable carbohydrate that aids in the differentiation of E. coli O157:H7 from other E. coli strains; non-fermenters (O157:H7) will remain colorless, while sorbitol-fermenters will turn pink. Bile salts and Crystal violet act as selective agents to inhibit the growth of Gram-positive organisms. Neutral red is a pH indicator.
Gelatin Peptone 17.0 g
Casein Peptone 1.5 g
Peptic Digest of Animal Tissue 1.5 g
Sorbitol 10.0 g
Bile Salts 1.5 g
Sodium Chloride 5.0 g
Neutral Red 0.03 g
Crystal Violet 0.001 g
Agar 13.5 g
Total 50.0 g/L
