The Abraxis Microcystins-ADDA SAES ELISA is an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent* detection of Microcystins and Nodularins in water samples.
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The Abraxis Microcystins-ADDA SAES ELISA is an enhanced sensitivity immunoassay for the quantitative and sensitive congener-independent* detection of Microcystins and Nodularins in water samples. The test is an indirect competitive ELISA for the congener-independent detection of Microcystins and Nodularins. It is based on the recognition of Microcystins, Nodularins, and their congeners by specific biotinylated antibodies. Toxin, when present in a sample, and a Microcystins-protein analog immobilized on the plate compete for the binding sites of the biotinylated anti-Microcystins/Nodularins antibodies in solution. The plate is then washed and a streptavidin-HRP is added. After a second washing step and addition of the substrate solution, a color signal is generated. The intensity of the blue color is inversely proportional to the concentration of Microcystins present in the sample. The color reaction is stopped after a specified time and the color is evaluated using an ELISA reader. The concentrations of the samples are determined by interpolation using the standard curve constructed with each run.
