This lateral flow strips (LFS) test kit is intended to be used for quantitative detection of CP4 EPSPS protein in Roundup ready (RUR)® bulk grain soybean or canola samples. The sensitivity of these strips for soybean is 0.05% (i.e. detects one Roundup Ready soybean in 2000 conventional soybeans) & for canola is 0.1% (i.e. detects one Roundup Ready canola kernel in 1000 conventional canola kernel).
The total incubation time of the assay is 5 minutes.
Details
When the LFS is placed in the sample extract, the CP4 EPSPS protein present in the sample extracts binds to the antibody labelled with small volume of gold nanoparticles and the complex moves upward by capillary action. The complex then binds to the antibody coated on the test line resulting in pink/purple color test line. As the complex moves further up, it binds to the control line resulting in pink/purple color control line. In absence of CP4 EPSPS protein, the test line does not appear as no complex binds to the test line while control line turns pink/purple color indicating validity of test protocol.
The intensity of test line can be used to quantitatively measure the amount of CP4 EPSPS present in the grain samples. The strips may then be scanned and the results are interpreted quantitatively with the help of RapidScan ST5 Lateral Flow Reader.
Note: The standard curve for the quantitative determination of CP4 EPSPS was derived from reference
material. Actual field expression may vary.
Kit Specifications
SKU
EAID 101