Cry1Ac/Ab ELISA Kit

An antibody specific to the Cry1Ac/Ab protein molecule is immobilized on the microwell plates and second antibody specific for Cry1Ac/Ab molecule is conjugated with horse radish peroxidase ( HRP). When the sample extracts are added to the microtiter wells, the Cry1Ac/Ab from the sample binds to the antibody immobilized in the well. This binding is subsequently detected by addition of enzyme-labeled antibody. After a washing step, substrate is added. The enzymatic reaction and development of color are proportional to the amount of Cry1Ac/Ab present in the sample. The reaction is terminated by the addition of stop solution. Absorbance is then measured on a plate reader.