PAT/Bar ELISA test kit is intended to be used for the qualitative detection of Liberty Link PAT/Bar protein in individual leaf/seed samples. The kit detects phosphinothricin acetyltransferase enzyme (PAT) coded for by the Bar gene in leaf or seed. The kit can be used for canola, rice, cotton and mustard leaf or seed sample.
An antibody specific to the PAT/Bar protein molecule is immobilized on the microwell plates and second antibody
specific for PAT/Bar molecule is conjugated with horse radish peroxidase (HRP).
When the sample extracts are added to the microtiter wells, the PAT/Bar protein from the sample binds to the
antibody immobilized in the well. This binding is subsequently detected by the addition of an enzyme-labeled antibody.
After a washing step, the substrate is added. The enzymatic reaction and development of color are proportional to the
amount of PAT/Bar protein present in the sample. The reaction is terminated by the addition of stop solution.
Absorbance is measured on a plate reader.
