PAT/Pat ELISA Kit

An antibody specific to the PAT/pat protein molecule is immobilized on the microwell plates and second antibody specific for PAT/pat molecule is conjugated with horse radish peroxidase (HRP). When the sample extracts are added to the microtiter wells, the PAT/pat protein from the sample binds to the antibody immobilized in the well. This binding is subsequently detected by the addition of the enzyme-labeled antibody. After a washing step, the substrate is added. The enzymatic reaction and development of color are proportional to the amount of PAT/pat protein present in the sample. The reaction is terminated by addition of stop solution. Absorbance is measured on a plate reader.