The VIRType SARS-CoV-2 (L452R) kit is designed for SARS-CoV-2 variant detection carrying L452R mutation using Real-time PCR from food and environmental surfaces and wastewater.
For Research Use Only!
Details
The new SARS-CoV-2 variants often carrying multiple spike protein mutations as well as mutations in other genomic regions. The spike protein is key for viral entry and for vaccine development. Therefore, specific identification of theses variants is critical.
The VIRType SARS-CoV-2 (L452R) kit detects one single point mutation which result in the exchange of the amino acid Leucine to Arginine in the spike protein at position 452.
The VIRType SARS-CoV-2 (L452R) kit provides all reagents for the rapid detection of the California lineages B.1.429 and B.1.427 as well as lineage B.1.526.1 and B.1.617 on food and environmental surfaces as well as wastewater via RT-qPCR. Single point mutation referring to the L->R amino acid exchange specific for those variants is detected in the FAMTM channel. As a reference, the respective wild type RdRP-gene region is detected in the HEXTM channel helping to confirm the presence of SARS-CoV-2.
The kit is validated for use with the Agilent AriaMxTM, Bio-Rad CFX96 TouchTM and the CFX96 TouchTM Deep Well PCR platforms.
It is not intended for clinical diagnostics and should therefore be regarded as “For Research Use Only”.
Kit Specifications
SKU
5728401601